Efficacy of Alpha Glucosidase Inhibitor from Marine Actinobacterium in the Control of Postprandial Hyperglycaemia in Streptozotocin (STZ) Induced Diabetic Male Albino Wister Rats

The current study was carried out to evaluate the in-vitro and in-vivo efficiency of alphaglucosidase inhibitor of marine actinobacteria in the control of postprandial hyperglycaemia.Soil samples were collected from salterns, coastal area in Kothapatnam, Ongole, AndhraPradesh, India. Among the actinobacterial isolates tested for yeastα-glucosidase inhibitoryactivity, only three isolates showed prominent inhibition. The patient isolate was selectedand identified as Streptomyces coelicoflavus SRBVIT13 using 16S r-RNA gene sequencing.In in-vitro studies, the chloroform extract of Streptomyces coelicoflavus SRBVIT13 showedsignificant enzyme inhibitory activity against yeast and mammalian α-glucosidaseenzymes.In animal studies, the oral ingestion of chloroform extract (600 mg/kg) of S. coelicoflavusSRBVIT13 in maltose and sucrose loaded diabetic rats, showed significant regulation ofpostprandial blood glucose by 82.25% and a 77.25% reduction, respectively. The leadcompound from S. coelicoflavusSRBVIT13 was isolated, purified, characterized, and identifiedby stranded analytical techniques as 2-t-butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid, t-butyl ester. The results obtained in the present study are promising and thebioactive compound from S. coelicoflavusSRBVIT13 may be considered as a potential agent inregulating the postprandial hyperglycaemia.